Production of isopropanol by metabolically engineered <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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Authors: | Toru Jojima Masayuki Inui Hideaki Yukawa |
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Institution: | (1) Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizugawa Kyoto, 619-0292, Japan |
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Abstract: | A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA)
transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary–secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production
by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing
pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol
production by genetically engineered microorganism under aerobic culture conditions. |
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Keywords: | Isopropanol Escherichia coli Clostridium acetobutylicum Clostridium beijerinckii Metabolic engineering |
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