Rapid detection of dilute lipopolysaccharide antigens in large volumes using polymyxin-coated polyester cloth |
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| Authors: | Button Lori Yamazaki Hiroshi |
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| Institution: | (1) Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Ontario, Canada, K1S 5B6 |
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| Abstract: | A segment of polymyxin-coated polyester cloth was placed onto a blotting pad and 10 mL of a dilute Salmonella lipopolysaccharide (LPS) sample was passively pipetted through the cloth segment (taking about 40 s). The LPS concentrated on the segment was assayed by immunoreaction with rabbit-anti LPS antibody and anti-rabbit-peroxidase conjugate (taking about 60 s), followed by colorimetric measurement of peroxidase. This rapid assay increased the detectability of Salmonella about 1,000 times. |
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