Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers |
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| Authors: | Ata Allah Sharafi Asad Asadi Abkenar Ali Sharafi Mohammad Masaeli |
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| Affiliation: | 1.Biotechnology Incubator Center of National Institute of Genetic Engineering and Biotechnology,Novin Giti Gene Biotech. Company (NGene Biotech. Co.),Tehran,Iran;2.Branch of North Region of Iran,Agricultural Biotechnology Research Institute of Iran (ABRII),Rasht,Iran;3.Zanjan Pharmaceutical Biotechnology Research Center, School of Pharmacy,Zanjan University of Medical Sciences,Zanjan,Iran;4.Pharmaceutical Biotechnology Department, School of Pharmacy,Zanjan University of Medical Sciences,Zanjan,Iran |
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| Abstract: | Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime “witches’ broom disease”. |
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