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Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis
Authors:Ursula K Schuch  Anita N Azarenko  Leslie H Fuchigami
Institution:(1) Department of Horticulture, Agriculture and Life Sciences Bldg., Oregon State University, 97331 Corvallis, OR, USA;(2) Present address: Department of Botany and Plant Sciences, University of California, 92521-0124 Riverside, CA, USA
Abstract:Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with 13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.Abbreviations FW fresh weight - IAA indole 3-acetic acid - HPLC high performance liquid chromatography - GC-MS-SIM gas chromatography-mass spectrometry selected ion monitoring - NAA naphthalene acetic acid - IBA indole butyric acid
Keywords:Coffea arabica  flower bud dormancy  water stress
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