Radioimmunoassay of human plasma corticosterone: method, measurement of episodic secretion and adrenal suppression and stimulation |
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Authors: | C J Nabors C D West D K Mahajan F H Tyler |
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Affiliation: | 1. Department of Anatomy and Radiobiology Laboratory and Department of Medicine, Laboratory for the Study of Hereditary and Metabolic Disorders University of Utah College of Medicine Salt Lake City, Utah 84132 U.S.A.;2. Clinical Research Center University of Utah College of Medicine Salt Lake City, Utah 84132 U.S.A. |
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Abstract: | A radioimmunoassay for human plasma corticosterone has been developed. Antisera were obtained by immunizing rabbits with corticosterone-21-hemisuccinate-BSA. An antiserum titer of 1:4000 was used for standard curves ranging from 0–1000 pg. Interfering steroids were removed from plasma extracts by paper chromatography. Plasma blanks obtained from adrenalectomized or Addisonian patients ranged from 29 to 42 ng/dl. Recovery of radioactive corticosterone through the entire method was 67.6 ± 5.2%. The coefficient of variation within assays was 19% and between assays 13%. The average 8 a.m. value in males was 396 ± 228 ng/dl and in females it was 655 ± 271 ng/dl. Corticosterone was found to be secreted episodically, in parallel with cortisol. Secretion of this steroid was suppressed by dexamethasone and stimulated by ACTH infusion. |
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