The Development and Stability of the Genus <Emphasis Type="Italic">Bacteriodes</Emphasis> from Human Gut Microbiota in HFA Mice Model |
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Authors: | Jing Yuan Benhua Zeng Rong Niu Huan Tang Wenxia Li Zhixue Zhang Hong Wei |
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Institution: | (1) Department of Laboratory Animal Science, College of Basic Medical Sciences Third Military Medical University, 30 Gaotanyan Street, Chongqing, 400038, China; |
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Abstract: | Human flora-associated (HFA) mice are frequently applied in studying the ecology and metabolism of human gut microbiota. However,
the development and stability of the genus Bacteriodes, a prominent bacteria group of human gut microbiota, in HFA mice have not yet fully been examined. In this study, PCR-denaturing
gradient gel electrophoresis (DGGE) analysis was employed to monitor the Bacteriodes community in the fecal microbiota of six HFA Kunming mice during a period of 3 weeks. Based on the DGGE banding patterns,
the majority of prominent bands in the HFA mice DGGE profile were also typical bands in the human DGGE profile, despite the
absence of three bands (corresponding to two different B. thetaiotaomicron strains and one B. intestinalis strain) from the human DGGE profile. The Dice coefficient of similarity for the fecal microbiota of HFA mice in comparison
to the human donor sample ranged between 74 ± 6% and 81 ± 7%. The phylogeny of bands in the DGGE profile showed that the dominant
Bacteriodes species in the fecal microbiota of HFA mice were B. thetaiotaomicron, representing 66.7% of all bands. Our results indicate that the genus Bacteriodes in the fecal microbiota of HFA mice was selected from the human donor and could remain relatively stable over time. |
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