Analysis of Bacterial Community Composition of a Spring Water from the Western Ghats,India Using Culture Dependent and Molecular Approaches |
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Authors: | Arunachalam Ruckmani Tapan Chakrabarti |
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Institution: | (1) Microbial Type Culture Collection & Gene Bank, Institute of Microbial Technology, Sector 39A, Chandigarh, India;(2) Microbial Culture Collection, National Centre for Cell Science, Pune University campus, Ganeshkhind, Pune, India |
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Abstract: | Cultivation based and culture independent molecular approaches were used to characterize the composition and structure of
bacterial community from a natural warm spring in the Western Ghats, a biodiversity ‘hotspot’. Dilution plating was done on
three types of media with varying nutrient levels. Relatively nutritionally poor medium supported growth of highest number
of bacteria (4.98 × 103 ml−1) compared to nutritionally rich media. On the basis of different morphological features on the plate, 62 aerobic and heterotrophic
bacterial strains were isolated and their 16S rRNA genes were sequenced and analyzed. On the basis of sequence similarity
these isolates were found to be distributed in 21 different genera belonging to Proteobacteria (58%) followed by Firmicutes (26%), Actinobacteria (13%) and Bacteroidetes (3%). Amplification of 16S rRNA gene of the community DNA using eubacterial primers, followed by cloning and sequencing revealed
that predominant members of the habitat belong to the phylum Cyanobacteria (60%) followed by Proteobacteria (19.5%), Bacteroidetes (6.67%), Actinobacteria (4.4%) and Firmicutes (2.2%) and small ribosomal subunit of a plastid (of Chlorophyta, 2.2%). |
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