The alm1+ gene from Schizosaccharomyces pombe encodes a coiled-coil protein that associates with the medial region during mitosis |
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Authors: | Jiménez M Petit T Gancedo C Goday C |
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Affiliation: | (1) Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas. Velázquez 144, 28006 Madrid, Spain E-mail: Claragoday@cib.csic.es Tel.: +34-91-5611800; Fax: +34-91-5627518, ES;(2) Instituto de Investigaciones Biomédicas “Alberto Sols”, C.S.I.C-UAM, Arturo Duperier 4, 28029 Madrid, Spain, ES |
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Abstract: | We have isolated a cDNA that encodes a 142-kDa protein by immunoscreening of a Schizosaccharomyces pombe expression library with a new antibody, mAb8, that reveals spindle poles and equatorial ring-like structures in several organisms. This cDNA encodes a putative protein which we termed Alm (for abnormal long morphology). The protein is predicted to be a coiled-coil protein, containing a central α-helical domain flanked by non-helical terminal domains. Immunofluorescence analysis showed that Alm1 is localized in the medial region of the cell from anaphase to the end of cytokinesis. Cells carrying an alm1::ura4 + disruption are viable and exhibit an elongated morphology. Homozygous alm1::ura4 + diploids sporulated normally but the spores did not germinate. Spores that have inherited the disruption allele from a heterozygous alm1 + / alm1::ura4 + diploid germinated but generated smaller colonies. We propose that Alm1 participates in the structural organization of the medial region in S. pombe. Received: 9 April 1999 / Accepted: 22 October 1999 |
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Keywords: | Schizosaccharomyces pombe Mitosis Medial region Coiled-coil protein |
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