西藏高原人群铁调素基因hamp密码子偏好性分析

目的:通过对西藏高原人群及平原人群、恒河猴等其他5种物种的密码子使用进行分析,从而得出西藏高原人群铁调素基因(hamp)的密码子偏好性。方法:采用PCR技术获得西藏高原人群铁调素全基因序列,利用在线软件CodonW进行密码子偏好性分析,通过在线软件PredictProtein以及Signal P等软件进行西藏高原人群铁调素基因的结构分析,比较与GenBank数据库中选取的平原人群、恒河猴等其他5种物种的密码子偏好性的差异。结果:西藏高原人群的铁调素基因全长为2681 bp,由3个外显子和2个内含子组成,编码84个氨基酸的铁调素前体肽,包含信号肽、中间肽与成熟肽,其密码子偏好性与平原人群、恒河猴等其他5个物种的密码子偏好性均有不同程度的差异。结论:西藏高原人群铁调素基因hamp密码子偏好性与其他物种的密码子偏好性均有不同程度的差异。

Analysis of the Codon Bias of Hamp in the Tibetan

ABSTRACT Objective: The codon usage of hamp in the Tibetan and other species which include the plain and the rhesus monkey were analyzed to find the codon bias of hamp in the Tibetan. Methods: The PCR technology was used for the full genetic sequence of the Tibetan, using online software CodonW to analysis codon bias, other online software that PredictProtein and Signal P were used to analy- sis the structure of hamp of the Tibetan and comparing the codon bias of the Tibetan with other 5 species which were selected from the GenBank database. Results: The genetic sequence of hamp in Tibetan was 2681 bp, the protein structure with a signal peptide but no transmembrane domain, there was significant difference between the Tibetan and other species in the codon bias. Conclusion: There was significant difference between the Tibetan and other species in the codon bias.