长链非编码RNA HOXA11-AS在胶质瘤中的表达及其临床意义的研究

目的:探讨长链非编码RNA HOXA11-AS在胶质瘤组织中的表达以及与胶质瘤患者临床预后的相关性。方法:首先,应用RT-PCR法检测人胶质瘤组织以及正常脑组织中HOXA11-AS的表达情况;其次,分析HOXA11-AS的表达水平与胶质瘤患者临床病理学特征之间的关系;最后,探讨HOXA11-AS的表达水平与胶质瘤患者预后之间的相关性。结果:RT-PCR显示,较之于正常脑组织(1.00±0.17),HOXA11-AS在胶质瘤组织(3.89±0.34)中的表达水平显著升高(P0.001),且随着肿瘤学分级的增高,HOXA11-AS的表达水平也随着升高(Grade Ⅰ-Ⅱ, 2.96±0.21 vs. Grade Ⅲ-Ⅳ, 4.83±0.50, p=0.003)。x~2检验提示HOXA11-AS表达水平与胶质瘤患者的肿瘤学分级、KPS评分以及患者的复发情况具有显著相关性,而与患者的年龄、性别、肿瘤大小等无相关性。Kaplan-Meier分析患者生存率,结果显示,HOXA11-AS低表达组患者的生存率明显高于HOXA11-AS高表达组患者,差异具有统计学意义(P0.001)。最后,我们的研究结果发现,HOXA11-AS的高表达水平、肿瘤学分级的增高、KPS评分80分均为影响胶质瘤患者预后的独立危险因素(P0.05)。结论:HOXA11-AS与胶质瘤患者预后密切相关,且为预测患者预后的独立因素。

The Expression of Long Non-coding RNA HOXA11-AS and Its Clinical Significance in Glioma

ABSTRACT Objective: To investigate the expression level of long non-coding RNA HOXA11-AS in glioma tissues and its correla- tion with clinical prognosis of glioma patients. Methods: First, we used RT-PCR to detect the expression level of HOXA11-AS in glioma tissues and normal brain tissues. Secondly, the relationship between HOXA11-AS expression and clinicopathological features of glioma patients was analyzed. Finally, we investigate the correlation between the expression level of HOXA11-AS and the prognosis of glioma patients. Results: The results from RT-PCR showed that the expression level of HOXA11-AS was significantly elevated in glioma tissues (3.89±0.34), when compared to that in normal brain tissue (1.00±0.17, P<0.001), and the expression level in glioma tissues was much higher in high-grade glioma than that in low-grade glioma (Grade I-II, 2.96±0.21 vs. Grade III-IV, 4.83±0.50, p=0.003). x2 analysis re- vealed HOXA11-AS expression was closely related to tumor grade, KPS score and tumor recurrence, whereas, there was no relationship with age, sex and tumor size. In addition, Kaplan-meier analysis showed that the survival rate of patients in HOXA11-AS low expression group was much higher than that in HOXA11-AS high expression group, and the difference was statistically significant (P<0.001). Finally, our results showed that the high expression level of HOXA11-AS, the advance of tumor grade, and KPS<80 were all independent risk factors affecting the prognosis of glioma patients(P<0.05). Conclusion: HOXA11-AS is closely related to the prognosis of glioma pa- tients and is an independent factor for predicting prognosis of patients.