乙肝患者肝组织乙型肝炎病毒cccDNA和血清MIF表达的相关性研究

目的:探讨乙肝患者肝组织乙型肝炎病毒(hepatitis B virus,HBV)共价闭合环状DNA(covalently closed circular DNA,cccDNA)和血清巨噬细胞移动抑制因子(Macrophage migration inhibitory factor,MIF)的表达相关性。方法:选择2016年2月到2016年7月在我院诊治的乙肝患者144例作为乙肝组,同期选择体格检查健康者144例作为对照组,采集所有入选者的血清样本,检测血清MIF、谷丙转氨酶(Glutamic pyruvic transaminase,ALT)、谷草转氨酶(Glutamic pyruvic aminotransferase,AST)、总胆红素(total bilirubin,TBIL)的表达,并对乙肝组患者肝组织HBV cccDNA采用荧光定量PCR技术检测表达分析,直线相关分析乙肝组的血清HBV cccDNA表达量与血清ALT、TBi L、AST、MIF含量相关性。结果:乙肝组的血清MIF、ALT、TBi L、AST含量均明显高于对照组(P0.05)。乙肝组的肝组织HBV cccDNA阳性率为54.17%(78/144)。直线相关分析显示乙肝组的肝组织HBV cccDNA表达量与血清ALT、TBi L、AST、MIF含量均呈现明显正相关性(P0.05)。结论:乙肝患者体内血清MIF水平明显升高,伴肝组织HBV cccDNA的表达也升高,两者存在明显的正相关性。因此检测血清MIF水平有助于评估乙肝患者HBV的感染情况。

Relationship between the Eexpression of Liver Tissue Hepatitis B Virus cccDNA and Serum MIF in Patients with Hepatitis B

ABSTRACT Objective: To investigate the correlation of the expression of liver tissue hepatitis B(HBV) virus cccDNA with serum macrophage migration inhibitory factor (MIF) in patients with hepatitis B. Methods: From February 2016 to July 2016, 144 cases of patients with hepatitis B in our hospital were selected as the hepatitis B group, and the other 144 cases of healthy controls undergoing physical examination were selected as the control group at the same time. The expression of HBV cccDNA in the liver tissue of patients with hepatitis B was detected by real-time quantitative PCR. The correlation of HBV cccDNA expression and serum ALT, TBiL, AST and MIF levels in hepatitis B group was analyzed by linear correlation analysis. Results: The serum levels of MIF, ALT, TBiL and AST in the hepatitis B group were significantly higher than those in the control group (P<0.05). The positive rate of HBV cccDNA in liver tissue of hepatitis B group was 54.17%(78/144). The linear correlation analysis showed that the expression of HBV cccDNA were positively correlated with the serum ALT, TBiL, AST and MIF levels (P<0.05). Conclusion: The level of serum MIF was positively correlated with the expression of HBV cccDNA in the liver tissue of patients with hepatitis B, Therefore, the detection of serum MIF level can contribute to assess the HBV infection in hepatitis B patients.