| 植物N-糖链检测技术研究进展 |
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| 作者姓名： | 何金霞 贾晓晨 王文霞 胡建恩 尹恒 |
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| 作者单位： | 1.大连海洋大学食品科学与工程学院, 辽宁 大连 116023;
2.中国科学院大连化学物理研究所, 大连市糖类农用制剂工程研究中心; 辽宁省碳水化合物研究重点实验室, 辽宁 大连 116023 |
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| 基金项目： | 国家自然科学基金项目（31672077）；辽宁省自然科学基金项目（201602742）；陕西省重点研发计划项目（2017TSCXL-NY-02-05）资助。 |
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| 摘 要： | N-糖基化作为一种重要的蛋白质翻译后修饰，在胚胎发育、癌症发生发展及免疫防御等诸多复杂的生命活动中发挥着关键作用。近年来，基于质谱的N-糖链的检测及其定量研究在动物方面取得了显著进展，相比之下，植物N-糖基化及N-糖链检测的相关研究要远远滞后，这也是制约植物糖生物学研究发展的关键瓶颈问题之一。对蛋白质N-糖链的释放、定量策略、可视化检测及其在植物中的应用进展进行了归纳总结，以期为指导后续植物N-糖链及N-糖组的定性定量检测提供参考。
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| 关 键 词： | N-糖基化 N-糖链 N-糖组 质谱 植物糖生物学 |
| 收稿时间： | 2018-10-15 |
Progress on Detection Technology of N-glycan in Plant |
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| Authors: | HE Jinxia JIA Xiaochen WANG Wenxia HU Jianen YIN Heng |
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| Institution: | 1.School of Food Science and Engineering, Dalian Ocean University, Liaoning Dalian 116023, China;
2.Liaoning Provincial Key Laboratory of Carbohydrates; Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning Dalian 116023, China |
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| Abstract: | N-glycosylation is the most prevalent post-translational modification of proteins, and plays an important role in multiple biological processes, such as embryonic development, cancer development and immune defense. In recent years, the strategies for mass spectrometry-based identification and quantification of N-glycans have made significant progresses in animals. In contrast, the studies on detection of N-glycosylation and N-glycans in plants have lagged far behind, and this is also one of the key bottlenecks in the study of plant glycobiology. The release, quantitative strategy, visual detection of N-glycans and their application progresses in plants were reviewed in order to provide references for guiding the qualitative and quantitative detection of N-glycan and N-glycome in plants. |
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| Keywords: | N-glycosylation N-glycan N-glycome mass spectrometry plant glycobiology |
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