速生桉叶水溶提取物对HepG2细胞增殖及凋亡的影响

目的:探讨速生桉叶水溶提取物对HepG2细胞的抑制增殖作用及凋亡的影响。方法:采用MTT法检测速生桉叶水溶提取物对HepG2细胞增殖抑制作用;采用流式细胞仪Annexin V-FITC/PI检测速生桉叶水溶提取物处理后HepG2细胞凋亡的情况。结果:MTT分析显示,当细胞培养24 h时,稀释5、10倍HepG2细胞抑制率分别为(47.32±1.11)%、(15.76±3.50)%;当细胞培养48 h时,稀释5、10倍HepG2细胞抑制率分别为(44.13±10.93)%、(25.93±8.37)%;当细胞培养72 h时,稀释5、10倍HepG2细胞抑制率分别为(59.47±6.90)%、(41.02±4.27)%。流式细胞术结果显示,速生桉叶水溶提取物稀释30倍时可诱导31.03%的HepG2细胞进入早期凋亡阶段,随着稀释倍数的减少被诱导进入凋亡阶段的细胞数目逐渐升高。另外,随着水溶提取物作用时间的延长,进入凋亡阶段的细胞数目也逐渐升高。结论:速生桉叶水溶提取物对HepG2细胞增殖与凋亡具有量-效、时-效关系。

Effects of Leaf Water Extract from Fast-Growing Eucylyptus Robusta on Proliferaton and Apoptosis of Human Heptoma HepG2 Cells

ABSTRACT Objective: To investigate the effects of the leaf of Fast-growing eucylyptus extract on the proliferation and apoptosis of human hepatoma HepG2 cells. Methods: MTT assay was used to measure the leaf of Fast-growing eucylyptus extract on HepG2 cells activity. The cell apoptosis of HepG2 cells were determined by flow cytometry Annexin V-FITC/PI. Results: As the results of MTT showed, HepG2 cells were cultured for 24 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were (47.32±1.11) % and (15.76 ±3.50) %, respectively. When HepG2 cells were cultured for 48 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were respectively (44.13±10.93) % and (25.93±8.37) %. When HepG2 cells were cultured for 72 h, the inhibition rate of HepG2 cells diluted 5 and 10 times was (59.47±6.90) % and (41.02±4.27) %. The flow cytometry showed that after incubated with the leaf of fast-growing eucylyptus extract for diluted 30 times, the early of apoptosis were 30.03%. The ratios of apoptotic increased with concentration diluted times decreased, and it was in a time-dependent manner. Moreover, the ratios of apoptotic increased with the time prolonged of the leaf of fast-growing eucylyptus extract, and it was in a dose-dependent manner. Conclusion: The leaf of fast-growing eucylyptus extract can inhibit proliferation and induced apoptosis on HepG2 Cells in a time-and dose-dependent manner.