Effect of various methods of immobilization on the stability of a microbial biosensor for surfactants based onPseudomonas rathonis T |
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| Authors: | I. N. Semenchuk L. A. Taranova A. A. Kalenyuk P. V. Il’yasov A. N. Reshetilov |
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| Affiliation: | (1) Dumanskii Institute of Colloid Chemistry and Chemistry of Water, National Academy of Sciences of Ukraine, Kiev, Ukraine;(2) Institute of Sorption and Problems of Endoecology, National Academy of Sciences of Ukraine, Kiev, Ukraine;(3) Pushchino State University, pr. Nauki 3, 142292 Pushchino, Moscow oblast, Russia;(4) Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142292 Pushchino, Moscow oblast, Russia |
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| Abstract: | The operating and storage stability of a receptor element of an amperometric biosensor based on thePseudomonas rathonis strain T capable of degrading surfactants was tested. Microbial cells were immobilized by incorporation in gels (agar, agarose, and calcium-alginate), polyvinyl alcohol membrane, adhesion to Chromatographic paper GF/A, or by cross-linking induced by glutaric aldehyde. Incorporation of microbial cells in agar gel provides long-standing conservation of their activity and viability during measurements of high concentrations of surfactants and allows the receptor element of the biosensor to be rapidly recovered after measurements. |
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