Abstract: | Pithophora oedogonia (Mont.) Wittr. biomass in Surrey Lake, Indiana was greater in the littoral than in the pelagial region. Although mean soluble reactive phosphorus concentrations did not differ between the two areas, nitrate concentrations were almost six times higher in the cove than in the open water. Using laboratory cultures of Pithophora, the half saturation constant (Ks at 20° C relating filament growth to external concentrations of nitrate-nitrogen was determined to be 1.23 mg L?1 (=88 μM)and for phosphate-phosphorus, 0.1 mg L?1 (=3.22 μM). These values were used to calculate a NO3-N/PO4-P atomic ratio of 27.6. Comparison of this value with NO3-N/PO4-P ratios in Surrey Lake showed that nitrogen limiting conditions were prevalent in the open water section of the lake. Alkaline phosphatase and dark ammonia uptake analyses on field collected filaments from the shallow and deep water sections confirmed the hypothesis that nitrate is the major factor limiting growth of Pithophora in Surrey Lake. |