Assignment of cytosine N3 resonances in nucleic acids via intrabase three-bond coupling to amino protons |
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Authors: | Simon Rüdisser Jeffrey G. Pelton Ignacio Tinoco Jr. |
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Affiliation: | (1) Department of Chemistry, University of California and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720-1460, U.S.A. |
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Abstract: | Coherences were observed between 15N3 of cytosine and its trans amino proton (H42) using a modified gradient-based heteronuclear single quantum coherence (HSQC) pulse sequence optimized for three-bond proton-nitrogen couplings. The method is demonstrated with a 22-nucleotide RNA fragment of the P5abc region of a group I intron uniformly labeled with 15N. Use of intraresidue 15 N3-amino proton couplings to assign cytosine 15 N3 signals complements the recently proposed JNN HNN COSY [Dingley, A.J. and Grzesiek, S. (1998) J. Am. Chem. Soc., 120, 8293–8297] method of identifying hydrogen-bonded base pairs in RNA. |
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Keywords: | cytosine group I intron 1H-15N HSQC 15N NMR RNA |
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