Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker |
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Authors: | Hanaki Ken-ichi Momo Asami Oku Taisuke Komoto Atsushi Maenosono Shinya Yamaguchi Yukio Yamamoto Kenji |
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Affiliation: | Domestic Research Fellow, Japan Science and Technology Corporation, 4-1-8 Honmachi, Kawaguchi, Saitama 332-0012, Japan. hanaki@ri.imcj.go.jp |
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Abstract: | For the purpose of selecting the efficient dispersion condition of hydrophilic semiconductor quantum dots (QDs) in biological buffers, the dispersion of the QDs mixed with a serum albumin from 9 different species or an ovalbumin was compared by a fluorescence intensity analysis. The QDs mixed with sheep serum albumin (SSA) showed the highest fluorescence of all when the mixtures were dissolved in Dulbecco's MEM. QD/SSA complexes were accumulated in the endosome/lysosome of Vero cells and the fluorescence could be detected over a 5-day post-incubation period. The photostability of QD/SSA complexes associated with the endosomes was detectable, at least, 30 times as long as that of fluorescein-labeled dextran involved in endosomes. QD/SSA complex, therefore, can be used as a long-life and highly photostable endosome marker. |
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Keywords: | Albumin Cadmium selenide Dextran Endosome Fluorescein Lysosome Quantum dot Semiconductor |
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