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Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles
Authors:Claudia S O Silva  Ricardo P Baptista  Andrea M Santos  José M G Martinho  Joaquim M S Cabral  M Ângela Taipa
Institution:(1) Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal;(2) Centro de Química-Física Molecular, Complexo I, Instituto Superior Técnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal
Abstract:Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40°C but only 10% of initial hIgG at 25°C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5–10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.
Keywords:Adsorption  Human immunoglobulin G  Poly(methyl methacrylate)  Poly(N-isopropylacrylamide)  Thermosensitive polymers
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