A spectrophotometric method for determining the stream sample core diameter of a flow cytometer |
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| Authors: | P Blume |
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| Institution: | Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131. |
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| Abstract: | A simple, rapid, and accurate method is described for the determination of flow cytometer stream sample core diameters. A precisely formulated solution of potassium dichromate is introduced as a sample, the stream is collected, and its absorbance is measured using a spectrophotometer. This permits the calculation of the factor by which the original solution was diluted and, given the stream diameter, the sample core diameter. |
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