Dissection of GA 20-oxidase members affecting tomato morphology by RNAi-mediated silencing |
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Authors: | Jinghua Xiao Hanxia Li Junhong Zhang Rugang Chen Yuyang Zhang Bo Ouyang Taotao Wang Zhibiao Ye |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China;(2) College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China |
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Abstract: | GA 20-oxidase is a key enzyme involved in gibberellin (GA) biosynthesis. In tomato, the GA 20-oxidase gene family consists of three members: GA20ox1, GA20ox2, and GA20ox3. To investigate the roles of these three genes in regulating plant growth and development, we used RNA interference technology to generate three kinds of transgenic tomato plants with suppressed expression of each three individual genes. Suppression of GA20ox1 or GA20ox2 resulted in shorter stems, a decreased length of internodes, and small dark green leaves while plants with decreased expression of GA20ox3 had no visible changes on stems and leaves. The plants of the three transgenic lines can flower and set fruits normally, but the seeds from these plants germinated slower than that from the normal plants. Decreased levels of endogenous GAs were detected in the apex of the three transgenic lines. These results demonstrate that the three GA 20-oxidase genes play different roles in the control of plan vegetative growth, but show no effects on flower and fruit development.Equal contribution authors: J. Xiao and H. Li. |
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Keywords: | GA 20-oxidase Gibberellins Morphology Tomato |
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