Calpain-PKC Inter-Relations in Mouse Hippocampus: A Biochemical Approach |
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Authors: | Katia Touyarot Sylvie Poussard Catherine Verret Bernadette Aragon Patrick Cottin Xavier Nogues Jacques Micheau |
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Affiliation: | (1) ISTAB, Laboratoire de Biochimie et Technologie des Aliments, Université Bordeaux I, UA-INRA 429, Avenue des Facultés, 33405 Talence Cedex, France;(2) Laboratoire de Neurosciences Cognitives, Université Bordeaux I, CNRS-UMR 5807, Avenue des Facultés, 33405 Talence Cedex, France;(3) Laboratoire de Neurosciences Cognitives, Université Bordeaux I, CNRS-UMR 5807, Avenue des Facultés, 33405 Talence Cedex, France |
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Abstract: | In previous studies, we isolated and identified a -calpain/PKC complex from rabbit skeletal muscle. Here, we have used specific purification procedures in order to study the interactions between -calpain and PKC in mouse hippocampus, a brain structure implicated in memory processes. We observed that -calpain and conventional PKCs (, II and ) are co-eluted after anion exchange chromatography. In contrast to our previous results obtained on skeletal muscle, -calpain and PKC isoenzymes were dissociated after gel filtration chromatography. Furthermore, -calpain induced the proteolytic conversion of PKC, II, and into PKM, II, and with a preferential hydrolysis of PKC, a specific isoenzyme of the nervous system. Although the -calpain/PKC interactions in the hippocampus are quite different from skeletal muscle, our results however, point out the functional importance of these inter-relations. Moreover, as PKC has been involved in the biochemical events underlying learning and memory, the preferential relationship between -calpain and PKC promotes the importance of the role that -calpain could play in the cellular mechanisms of memory formation. |
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Keywords: | Calpain PKC PKM proteolysis hippocampus |
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