蒺藜中甾体皂苷对新生隐球菌生物膜形成的抑制作用

目的 观察蒺藜甾体皂苷类化合物TTS-12对新生隐球菌生物膜形成的影响,探讨其可能的作用机制.方法 光镜观察TTS-12对新生隐球菌生物膜生长形态的影响；MTT法观察TTS-12对新生隐球菌生物膜形成的影响；实时定量RTPCR观察不同浓度TTS-12对新生隐球菌细胞生物膜关键基因PMT4表达的影响.结果 经TTS-12处理的新生隐球菌生物膜结构更疏松,TTS-12可剂量依赖性地降低新生隐球菌生物膜生长动力学指标及PMT4基因表达水平(P＜0.01).结论 TTS-12可抑制新生隐球菌生物膜的形成.通过降低新生隐球菌PMT4基因表达可能是其抑制新生隐球菌生物膜的形成作用机制之一.

Inhibitory effects of Tribulus terrestris steroid saponin TTS-12 on building biofilm of Cryptococcus neoformans

Objective To investigate the influence of TTS-12,a steroid saponin from Tribulus terrestris,on building biofilm of Cryptococcus neoformans,and to explore the possible mechanism.Methods The inhibitory effects of biofilm formation of Cryptococcus neoformans by TTS-12 was observed by microscopy and assessed by MTT method.The expression of PMT4 mRNA was measured by real-time RT-PCR with different concentrations of TTS-12.Results Compared with control group,TTS-12 treatment showed inhibitory effects on the formation process,making the biofilm more loosened.The growth kinetics and PMT4 mRNA level in TTS-12-treated group were dose-dependently lower than those in the control group（P0.01）.Conclusions TTS-12 may has inhibitory effect on formation of Cryptococcus biofilm by PMT4 gene.