In vitro shoot regeneration from leaf explants of Roman Chamomile |
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Authors: | Echeverrigaray S Fracaro F Andrade LB Biasio S Atti-Serafini L |
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Institution: | (1) Institute of Biotechnology, University of Caxias do Sul, R. Francisco G. Vargas, 1130, CEP 95001-970, Caxias do Sul, RS, Brazil |
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Abstract: | Murashige and Skoog (1962) medium supplemented with 1.0 to 4.5 M of BA and 1.0 M of NAA induced adventitious bud formation and shoot development in leaf explants of Roman Chamomile. A higher number of adventitious buds was observed at the proximal end of the explants. Plantlets were replicated and multiplied on MS medium supplemented with 2.25 M of BA and 0.6 M of IAA. Plantlets were rooted on MS medium supplemented with 0.5 M of IBA and successfully weaned in vivo. The plants grew to maturity with high uniformity and no morphological signs of somaclonal variation. |
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Keywords: | Anthemis nobilis Compositae medicinal plant organogenesis |
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