Sequence comparison of the mitochondrial genomes of five brackish water species of the family Neritidae: Phylogenetic implications and divergence time estimation |
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Authors: | Jing Miao Jiantong Feng Xiaojuan Liu Chengrui Yan Yingying Ye Jiji Li Kaida Xu Baoying Guo Zhenming Lü |
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Institution: | 1. National Engineering Research Center for Facilitated Marine Aquaculture, Zhejiang Ocean University, Zhoushan China ; 2. Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou Guangdong, China ; 3. Marine Fishery Institute of Zhejiang Province, Key Laboratory of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Zhejiang Ocean University, Zhoushan China |
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Abstract: | Neritids are ancient gastropod species which can live in marine, brackish water, and freshwater environments. In this study, we sequenced and annotated the mitochondrial genomes of five brackish water neritids (i.e., Clithon corona, Clithon lentiginosum, Clithon squarrosum, Neritina iris, and Septaria lineata). The mitogenomes ranged from 15,618 to 15,975 bp, and all contain 13 protein‐coding genes (PCGs), 22 tRNA genes, and two rRNA genes, with a closed ring structure. We calculated the Ka/Ks values of all 13 PCGs of Neritidae species, all ratios are less than 1, under purification selection. Phylogenetic analysis of the 13 PCGs showed that Neritimorpha is a sister group with Vetigastropoda and Caenogastopoda, genus Clithon is a sister group with Neritina and Septaria. Estimation of divergence time for all species of Neritidae showed that the main differentiation of Neritidae occurred in Cenozoic period (65 Mya), C. corona and C. lentiginosum were differentiated in the Cenozoic Neogene, the other three species diverged in the Cenozoic Paleogene. These results will help to better understand the evolutionary position of Neritidae and provide reference for further phylogenetic research on Neritidae species. |
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Keywords: | divergence time mitogenome Neritid phylogenetic |
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