Expression and regulation of vitellogenin messenger RNA in the mosquito,Aedes aegypti |
| |
Affiliation: | 1. Department of Entomology Cornell University, Ithaca, NY 14853, U.S.A.;2. Department of Biochemistry, Cornell University, Ithaca, NY 14853, U.S.A. |
| |
Abstract: | Vitellogenin (yolk protein) gene expression in the mosquito was investigated at the level of mRNA using a subcloned fragment (403-1c) of the vitellogenin DNA derived from an Aedes aegypti genomic library. Message appeared 1–3 hr after a blood meal, peaked at 36 hr and was rapidly degraded thereafter. Fluctuations in levels of 20-hydroxyecdysone after a blood meal coincided with accumulation of vitellogenin message. Blood-fed, decapitated females injected with 5 μg of 20-hydroxyecdysone accumulated up to 75% of the message found in blood-fed controls. Fat bodies from non-blood-fed females incubated with physiological levels of 20-hydroxyecdysone and the juvenile hormone analog methoprene contained twice as much vitellogenin message as those incubated with 20-hydroxyecdysone alone. Methoprene alone had no effect. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|