Diagnosis of major helminth infections by ria detection of ecdysteroids in urine and serum |
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| Affiliation: | 1. Laboratory for Residue Control;2. Laboratory for Mastitis and Raw Milk Quality, and;3. Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Croatian Veterinary Institute, 10000 Zagreb, Croatia;;4. Laboratory for Analytical Chemistry and Residues, Croatian Veterinary Institute Križevci, 48260 Križevci, Croatia; and;5. Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia;1. Biomedical Sciences Department, Tshwane University of Technology, Arcadia Campus, Pretoria 0001, South Africa;2. Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida 1709, South Africa;3. Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2531, South Africa;4. Epidemiology, Parasites and Vectors, Agricultural Research Council - Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa |
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| Abstract: | Helminthiases can be detected by the analysis of ecdysteroids in the serum of man. This is concluded from the analysis of the serum from 38 patients infected with parasites belonging to the three systematic categories of Nematoda, Trematoda and Cestoda. In an ecdysteroid radioimmunoassay all but four patients had elevated levels of immuno-reactive material in their serum. Uninfected persons were essentially ecdysteroid free.Ecdysteroids were also detected in the serum, urine and cerebrospinal fluid of a patient with the tentative diagnosis of intracerebral cysticercosis. |
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