Propionate catabolism in the housefly Musca domestica and the termite Zootermopsis nevadensis |
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| Institution: | 1. Department of Botany, Panjab University, Chandigarh, India;2. I.K. Gujral Punjab Technical University, Chandigarh, India;3. Department of Biotechnology, Shaheed Udham Singh College of Engineering and Technology, Tangori, Mohali, India;1. Misaki Marine Biological Station, School of Science, The University of Tokyo, Misaki, Miura, Kanagawa, 238-0225, Japan;2. National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan;3. Laboratory of Ecological Genetics, Faculty of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan;4. Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan;1. Cardiff School of Biosciences, Cardiff University, CF10 3AX, Wales, UK;2. Cardiff School of Medicine, Cardiff University, CF14 4YS, Wales, UK;1. Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 252 42 Vestec, Czech Republic;2. Department of Zoology, Faculty of Science, Charles University, Viničná 7, 128 44 Prague 2, Czech Republic;1. The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Victoria, Australia;2. Department of Pharmacology and Therapeutics, University of Melbourne, Victoria, Australia;3. Klinik für Psychiatrie, Psychosomatik und Psychotherapie, Universitätsklinikum Frankfurt, Frankfurt am Main, Germany;4. Department of Psychiatry, School of Medicine, University of California San Diego, La Jolla, California;5. School of Nursing and Human Sciences, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin;6. Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland;7. Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland |
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| Abstract: | The catabolism of propionate was examined in the housefly Musca domestica (which does not contain detectable amounts of vitamin B12) and the termite Zootermopsis nevadensis (which contains large amounts of vitamin B12). The products from carbon-14 labeled propionate were separated by HPLC and radioactivity was determined by liquid scintillation counting. In vivo studies as a function of time showed that, in both species, products of 2-14C]propionate were acetate and 3-hydroxypropionate. 2-14C]Propionate was not efficiently converted to methylmalonate or succinate, as would occur in mammals. Studies with sub-cellular fractions in both species showed that only the mitochondrial fraction efficiently converted propionate to acetate. Radioactivity from 1-14C]propionate incubated with housefly mitochondria was recovered only in fractions corresponding to propionate and 3-hydroxypropionate. The data obtained are consistent with a metabolic pathway in which propionate is converted to 3-hydroxypropionate and then to acetate. The results presented here demonstrate this pathway in insects which have high vitamin B12 levels and undetectable vitamin B12 levels, suggesting that this may be a common pathway for propionate metabolism in insects. |
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