A study of the effect of heat shock and metal ions on protein synthesis in Neurospora crassa cells |
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| Affiliation: | 1. Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea;2. Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 406-772, South Korea;3. Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea;4. Pathology Team, National Fisheries Research & Development Institute, Busan 619-902, South Korea;5. Department of Marine Biotechnology, College of Liberal Arts and Sciences, Anyang University, Ganghwa 417-833, South Korea;6. Department of Life Science, College of Natural Sciences, Sangmyung University, Seoul 110-743, South Korea |
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| Abstract: | - 1.1. Neurospora cells were grown at 28°C for 14hr and subjected to heat shock (HS) at 48°C for 45 min. Protein synthesis profiles, monitored by labelling with [35S]methionine and one and two-dimensional electrophoresis, revealed nine heat shock proteins (HSPs).
- 2.2. Crossed-immunoelectrophoresis revealed five polypeptides in the shocked cell extracts that were not detectable in normal cells.
- 3.3. Synthesis of HSPs occurred rapidly during the shock treatment and ceased upon transfer to normal conditions. One of the HSPs—~43 K in size—may be a developmentally-regulated protein.
- 4.4. Metal ions—cadmium, zinc, manganese, copper—did not elicit a stress response when used alone but appeared to modulate the heat shock response.
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