In vitro Production of Halo Blight Inducing Toxin by Pseudomonas phaseolicola (Burkh.) Dowson |
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| Authors: | P S Bisen Anjana Sharma G P Agarwal |
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| Institution: | Department of Postgraduate Studies and Research in Biological Sciences, Rani Durgawati University, Jabalpur 482001 (M.P.) (India). |
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| Abstract: | Three pathogenic strains of Pseudomonas phaseolicola (strain 1 and 3 virulent and strain 5 weakly virulent) were tested for their toxic activity. All three strains produced detectable amounts of toxin in vitro. Cultural conditions and length of incubation greatly influenced toxin production. Maximum amount of toxin was produced at 20°C and pH 6.5. Glycerol served as the best carbon source and 1-cysteine as the best amino acid for toxin production. |
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