A co-culture system with preantral follicular granulosa cells in vitro induces meiotic maturation of immature oocytes |
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Authors: | Zhanbiao Li Pan Zhang Zhipeng Zhang Bo Pan Huhe Chao Lan Li Qingjie Pan Wei Shen |
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Institution: | (1) Laboratory of Germ Cell Biology, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China; |
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Abstract: | Development of technologies to mature oocytes in vitro is important for in vitro fertilization research. Here, we investigated
the ability of preantral follicular granulosa cells (PAGCs) to restrain apoptosis and to promote the growth and meiotic resumption
of immature murine oocytes in vitro. The oocytes of 55–65 μm derived from 12 to 14 days old juvenile mice were co-cultured
with PAGCs in vitro. The results showed that the oocytes co-cultured with PAGCs for 7 days grew faster and 14.6% of immature
oocytes were able to complete the first meiotic division and arrive at the MII stage. 71 oocytes co-cultured with PAGCs were
fertilized and 16 embryos were able to form morula-blastocysts. Following the co-culture of immature oocytes with/without
PAGCs for 7 days, the percentage of apoptotic oocytes were 33.5 and 51.4%, respectively (p < 0.01). Furthermore, the inhibition of apoptosis was communicated between oocytes and PAGCs through the GDF9-PI3 K-Akt signaling
pathway. In conclusion, the co-culture with PAGCs has a beneficial effect on the growth and maturation of immature oocytes. |
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