<Emphasis Type="Italic">In vitro</Emphasis> propagation of the chinese medicinal plant, <Emphasis Type="Italic">Dendrobium candidum</Emphasis> wall. ex lindl., from axenic nodal segments |
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Authors: | Yih-Juh?Shiau Satish?Manohar?Nalawade Chi-Ni?Hsia Vanisree?Mulabagal Email author" target="_blank">Hsin-Sheng?TsayEmail author |
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Institution: | (1) Department of Agronomy, Agricultural Research Institute, Wufong, 413 Taichung, Taiwan;(2) Institute of Biotechnology, Chaoyang University of Technology, Wufong, 413 Taichung, Taiwan |
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Abstract: | Summary
Dendrobium candidum Wall. Ex Lindl. is an important species used in the formulation of Shih-hu, a Chinese traditional medicine. An efficient
protocol for in vitro propagation of D. candidum using the axenic nodal segments of the shoots, originated from the in vitro germinated seedlings, was developed. The seeds from 120-d-old capsules after pollination were first germinated on half-strength
Murashige and Skoog (MS) basal medium supplemented with 30 g l−1 sucrose. After 4 mo., the seedlings were subcultured on a similar medium supplemented with 1 ml l−1 HYPONeX, 80 g l−1 potato homogenate and 2 g l−1 activated charcoal for further growth. Axenic nodal segments excised from 9-mo.-old seedlings were cultured on the medium
in the presence of 2 mg l−1 benzyladenine (BA) and 0.1 mg l−1 naphthaleneacetic acid (NAA). After 75 d, 73.2% of the explants gave rise to buds/shoots. The elongated shoots were rooted
on the medium containing 0.2 mg l−1 NAA and the plantlets were successfully acclimatized in soil. |
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Keywords: | axenic nodal segments Dendrobium candidum medicinal herb tissue culture |
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