KANK1对子宫内膜癌细胞增殖及迁移的影响

摘要 目的：研究KANK1在子宫内膜癌中的表达以及对子宫内膜癌细胞增殖及迁移的影响。方法：（1）TCGA数据库分析KANK1在子宫内膜癌中的表达和生存期分析。（2）采用实时荧光定量聚合酶链反应验证转染KANK1质粒的效果。采用Ishikawa和ECC1这两种子宫内膜癌细胞来探讨KANK1对子宫内膜癌的细胞周期和凋亡的影响。通过Western blot检测细胞周期相关蛋白的表达，以及流式细胞术检测细胞周期和凋亡水平。（3）通过Transwell小室实验和划痕实验检测细胞的侵袭和转移能力。结果：TCGA数据库分析发现KANK1在子宫内膜癌中低表达且与患者预后良好相关。过表达KANK1下调了Cyclin D1和Cyclin D2的蛋白水平，并将细胞周期阻滞在G1期。流式细胞术检测发现过表达KANK1组的细胞凋亡水平（Ishikawa：22.7%；ECC1：19.0%）比对照组（Ishikawa：18.1%；ECC1：15.3%）高，差异具有统计学意义。Transwell迁移和侵袭实验结果表明过表达KANK1组的子宫内膜癌细胞侵袭和转移能力减弱。结论：本研究证明了KANK1在子宫内膜癌中发挥抑癌作用。KANK1高表达与子宫内膜癌的预后良好成正相关。KANK1通过抑制癌细胞周期和促进肿瘤细胞凋亡发挥抑制子宫内膜癌增殖的作用。此外，KANK1抑制了子宫内膜癌的侵袭和转移。

Effect of KANK1 on Proliferation and Migration of Endometrial Cancer Cells

ABSTRACT Objective: To investigate the expression of KANK1 in endometrial cancer and its effect on the proliferation and migration of endometrial cancer. Methods: (1) TCGA database was used to analyze the expression of KANK1 and its survival analysis in endometrial cancer. (2) Real-time quantitative polymerase chain reaction was used to verify the effect of transfection of KANK1 plasmid. Ishikawa and ECC1 cells were used to investigate the effect of KANK1 on cell cycle and apoptosis of endometrial cancer cells. The expression of cell cycle-related proteins was detected by Western blot, and the cell cycle and apoptosis levels were detected by flow cytometry. (3) Cell invasion and metastasis ability were detected by transwell assay and wound healing assay. Results: TCGA database analysis showed that KANK1 was low expressed in endometrial cancer and correlated with good prognosis. Overexpression of KANK1 downregulated the protein levels of Cyclin D1 and Cyclin D2 and blocked the cell cycle in G1 phase. Flow cytometry showed that the apoptosis level of KANK1-overexpression group (Ishikawa: 22.7%; ECC1: 19.0%) was higher than that of the control group (Ishikawa: 18.1%; ECC1: 15.3%), and the difference was statistically significant. Transwell assay and wound healing assay showed that the invasion and metastasis ability of endometrial cancer cells overexpressing KANK1 was diminished. Conclusion: This study demonstrates that KANK1 plays an anti-tumor role in endometrial cancer. The high expression of KANK1 is positively related to the good prognosis of endometrial cancer. KANK1 inhibits the proliferation of endometrial cancer by inhibiting cancer cell cycle progression and promoting tumor cell apoptosis. In addition, KANK1 inhibits the invasion and metastasis of endometrial cancer.