Efficient Gene Knockout in Goats Using CRISPR/Cas9 System |
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| Authors: | Wei Ni Jun Qiao Shengwei Hu Xinxia Zhao Misha Regouski Min Yang Irina A Polejaeva Chuangfu Chen |
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| Institution: | 1. College of Life Sciences, Shihezi University, Shihezi, Xinjiang, China.; 2. Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah, United States of America.; 3. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.; NIH, United States of America, |
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| Abstract: | The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that CRISPR/Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts. Four genes were disrupted simultaneously in goat fibroblasts by CRISPR/Cas9-mediated genome editing. The single-gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live-born goats harboring biallelic mutations. The CRISPR/Cas9 system represents a highly effective and facile platform for targeted editing of large animal genomes, which can be broadly applied to both biomedical and agricultural applications. |
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