Analytic 3D Imaging of Mammalian Nucleus at Nanoscale Using Coherent X-Rays and Optical Fluorescence Microscopy |
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| Authors: | Changyong Song Masatoshi Takagi Jaehyun Park Rui Xu Marcus Gallagher-Jones Naoko Imamoto Tetsuya Ishikawa |
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| Affiliation: | 1.RIKEN SPring-8 Center, Sayo, Hyogo, Japan;2.RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, Japan;3.Department of Physics and Astronomy, University of California, Los Angeles, California;4.Institute of Integrative Biology, University of Liverpool, Liverpool, UK |
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| Abstract: | Despite the notable progress that has been made with nano-bio imaging probes, quantitative nanoscale imaging of multistructured specimens such as mammalian cells remains challenging due to their inherent structural complexity. Here, we successfully performed three-dimensional (3D) imaging of mammalian nuclei by combining coherent x-ray diffraction microscopy, explicitly visualizing nuclear substructures at several tens of nanometer resolution, and optical fluorescence microscopy, cross confirming the substructures with immunostaining. This demonstrates the successful application of coherent x-rays to obtain the 3D ultrastructure of mammalian nuclei and establishes a solid route to nanoscale imaging of complex specimens. |
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