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Understanding the molecular basis of plant growth promotional effect of Pseudomonas fluorescens on rice through protein profiling
Authors:Saveetha Kandasamy  Karthiba Loganathan  Raveendran Muthuraj  Saravanakumar Duraisamy  Suresh Seetharaman  Raguchander Thiruvengadam  Balasubramanian Ponnusamy  Samiyappan Ramasamy
Institution:(1) Defence Medical & Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, #09-01 Kent Ridge, 117510, Singapore;(2) Department of Anatomy (MD10), Venom and Toxin Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore, 117597, Singapore;(3) School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore
Abstract:

Background  

Heat stress (HS) and related illnesses are a major concern in military, sports, and fire brigadiers. HS results in physiologic responses of increased temperature, heart rate and sweating. In heat stroke, inflammatory response plays an important role and it is evidenced that turpentine (T) induced circulating inflammatory cytokines reduced survival rate and duration at 42°C. Here we report the alteration in the protein expression in liver cells upon HS with and without T treatment using two dimensional gel electrophoresis (2-DE), tryptic in-gel digestion and MALDI-TOF-MS/MS approaches
Keywords:
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