OsPRX2 contributes to stomatal closure and improves potassium deficiency tolerance in rice |
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| Authors: | Xiaohui Mao Yanmei Zheng Kaizhuan Xiao Yidong Wei Yongsheng Zhu Qiuhua Cai Liping Chen Huaan Xie Jianfu Zhang |
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| Affiliation: | 1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China;3. Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou Branch, National Center of Rice Improvement of China, Fuzhou 350003, China;4. National Engineering Laboratory of Rice, Fuzhou 350003, China;5. South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China |
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| Abstract: | Peroxiredoxins (Prxs) which are thiol-based peroxidases have been implicated in the toxic reduction and intracellular concentration regulation of hydrogen peroxide. In Arabidopsis thaliana At2-CysPrxB (At5g06290) has been demonstrated to be essential in maintaining the water-water cycle for proper H2O2 scavenging. Although the mechanisms of 2-Cys Prxs have been extensively studied in Arabidopsis thaliana, the function of 2-Cys Prxs in rice is unclear. In this study, a rice homologue gene of At2-CysPrxB, OsPRX2 was investigated aiming to characterize the effect of 2-Cys Prxs on the K+-deficiency tolerance in rice. We found that OsPRX2 was localized in the chloroplast. Overexpressed OsPRX2 causes the stomatal closing and K+-deficiency tolerance increasing, while knockout of OsPRX2 lead to serious defects in leaves phenotype and the stomatal opening under the K+-deficiency tolerance. Detection of K+ accumulation, antioxidant activity of transgenic plants under the starvation of potassium, further confirmed that OsPRX2 is a potential target for engineering plants with improved potassium deficiency tolerance. |
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| Keywords: | 2-Cys peroxiredoxins Potassium Stomatal closure Rice |
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