Species-specific peculiarities of functional reactions of the sodium pump to phosphorylation by protein kinase A |
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Authors: | N S Logvinenko F Cornelius |
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Institution: | 1. itInstitute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia 2. Faculty of Biophysics, ?rhus, Denmark
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Abstract: | The Na+,K+-ATPase isolated from shark rectal gland or pig kidney was inserted into liposomes and phosphorylated with cAMP-dependent
protein kinase without detergent. Stoichiometry of phosphorylation of α-subunit of the enzyme was 0.9 and 0.2 mol Pi/mol α-subunit of the pig kidney and shark gland, respectively. The phosphorylation of the shark Na+,K+-ATPase led to an increase in maximum of hydrolytic activity dependent on the cytoplasmic sodium concentration and the extracellular
activation with potassium ions. On the contrary, the phosphorylation of the sodium pump of the pig kidney did not produce
any significant functional effect. |
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