利用原子力显微镜识别成像

细胞膜和细胞内特异蛋白的有效定位与定性,对于了解细胞运动、移植和分化等机制及细胞之间的相互作用非常关键。原子力显微镜灵敏的力学性质在研究生物分子的相互作用和特定分子的免疫识别中得到了广泛的应用,在细胞表面的特异性分子的定位过程中,不像免疫荧光成像一样需要复杂的样品准备,更重要的是能有效地进行特异性和非特异性的识别,并对识别位点可视化。本文从分子识别、功能化探针、基于力-体积成像及与动态力学显微镜结合成像等模式方面,综述了原子力显微镜在生物应用中的识别成像。

Recognition Imaging by Atomic Force Microscopy

To mapping the distribution of receptor-associated banding protein on cell menbrance,it is great important to learn more about cell mobility,transplant,differentiation and the interaction between cells.Atomic force microscopy has evolved as an important technique in biological sciences due to its capability to visualize biomolecules at the nanometer scale in physiologically revelant aqueous environments.It is not require tedious sample preparation as mmunofluorescence assays,but it can distinguish the specific or non-specific recognition effectively,and visualize the location of specific molecules.This article reviewed the biophysical application of atomic force microscopy in recognition imaging from molecule recognition,functionalize the atomic force microscopy probe and two imaging mode force-volume mapping,topography and recognition imaging.