Immunological detection of arrestin, a phototransduction regulatory protein, in the cytosol of nucleated erythrocytes |
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Authors: | M Mirshahi F Borgese A Razaghi U Scheuring F Garcia-Romeu J P Faure R Motais |
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Affiliation: | INSERM U86, Institut Biomédical des Cordeliers, Paris, France. |
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Abstract: | Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extracts, these antibodies specifically recognized a protein having a molecular weight similar to that of retinal arrestin. Because the G-protein-mediated transduction systems, such as visual and beta-adrenergic systems, display a high degree of structural and functional homology, the presence of arrestin-like proteins in non-photosensitive cells suggests that these proteins are involved in the transduction of chemical signals, with a possible role in receptor desensitization. |
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