Introgression of Resistance to Powdery Mildew Conferred by Chromosome 2R by Crossing Wheat Nullisomic 2D with Rye |
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Authors: | Diao-Guo An Li-Hui Li Jun-Ming Li Hong-Jie Li Yong-Guan Zhu |
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Institution: | Centre of Agricultural Resources, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Shijiazhuang 050021, China;;Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing 100081, China;;Research Center for Eco-environmental Sciences, the Chinese Academy of Sciences, Beijing 100085, China;;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
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Abstract: | Using the nullisomic back-cross procedure, four wheat-rye chromosome substitution 2R (2D) lines with different agronomic performance, designated WR02-145-1, WR01-145-2, WR02-145-3, and WR02-145-4, were produced from a cross between 2D nullisomic wheat (Triticum aestivum L. cv. "Xiaoyan 6") and rye (Secale cereale L. cv. "German White"). The chromosomal constitution of 2n=42=21 in WR02-145 lines was confirmed by cytological and molecular cytogenetic methods. Using genomic in situ hybridization on root tip chromosome preparations, a pair of intact rye chromosomes was detected in the WR02-145 lines. PCR using chromosome-specific primers confirmed the presence of 2R chromosomes of rye in these wheat-rye lines, indicating that WR02-145 lines are disomic chromosome substitution lines 2R (2D). The WR02-145 lines are resistant to the powdery mildew (Erysiphe graminis DC. f. sp. tritici E. Marchal) isolates prevalent in northern China and may possess gene(s) for resistance to powdery mildew, which differ from the previously identified Pm7 gene located on chromosome 2RL. The newly developed "Xiaoyan 6"- "German White"2R (2D) chromosome substitution lines are genetically stable, show desirable agronomic traits, and are expected to be useful in wheat improvement. |
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Keywords: | genomic in situ hybridization nullisomic back-cross procedure PCR powdery mildew resistance wheat-rye chromosome substitution line |
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