脑桥呼吸调整中枢向延髓Botzinger复合体轴突投射的研究

实验在３３只成年猫上进行。Ｂｏｔｚｉｎｇｅｒ复合体内微量注入麦角辣根过氧化酶（ＷＧＡ－ＨＲＰ，３０－６０ｎｌ，５％，９例）后，在脑桥呼吸调整中枢结合臂外侧核及Ｋｏｌｌｉｋｅｒ－Ｆｕｓｅ核观察到大量ＨＲＰ标记神经元。在２０例动物中，检测了９１个在呼吸调整中枢记录到的呼吸神经元对电刺激Ｂｏｔｚｉｎｇｅｒ复合体的反应。其中１３个神经元（吸气性１１个，跨时相２个）可被逆行兴奋。实验结果表明，发自脑桥呼吸调整中枢神经元的轴突可投射到Ｂｏｔｚｉｎｇｅｒ复合体，这一投射通路可能与呼吸调节有关。

PROJECTIONS FROM PONTINE PNEUMOTAXIC CENTER TO MEDULLARY BOTZINGER COMPLEX IN CATS

Experiments were done on 33 adult cats. After injection of WGA-HRP (5%, 30- 60 nl ) into the Botzinger complex, labeled cells were observed in the lateralparabrachial nucleus and the Kolliker-Fuse nucleus. Injection into control sites failed tolabel the cells in the above two nuclei. Ninety-one respiratory related neurons recordedfrom the pneumotuxic center were tested by electrical stimulation of the Botzinger complex. Thirteen neurons (14. 3%, inspiratory 11, phase- sawnning 2 ) could be antidromically activated. Results of the present study showed that neurons in the pneumotoxic center sent axons to the Botzinger complex concerning respiratory control.