| 一种改进的超薄聚丙烯酰胺等电聚焦电泳实验 |
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| 引用本文： | 谢永芳,梁亦龙,张蕴,秦燕均,伍文勇,何元方.一种改进的超薄聚丙烯酰胺等电聚焦电泳实验[J].生物技术通讯,2006,17(1):70-71. |
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| 作者姓名： | 谢永芳 梁亦龙 张蕴 秦燕均 伍文勇 何元方 |
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| 作者单位： | 重庆邮电学院生物信息学院,重庆,400065 |
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| 基金项目： | 重庆邮电学院青年科学基金项目(2002-18A) |
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| 摘 要： | 目的:介绍一种自制、简易、高效的平板微型聚丙烯酰胺凝胶等电聚焦电泳方法。方法:将载玻片黏合作为制胶模具,剪滤纸制成上样芯子进行等电聚焦电泳。结果:电泳条带清楚,样品容量大,电泳时间短,操作性强。结论:此方法简单、有效、实用,所需凝胶量少,节约成本,值得推广。
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| 关 键 词： | 凝胶电泳 等电聚焦 等电点 |
| 文章编号： | 1009-0002（2006）01-0070-02 |
| 收稿时间： | 2005-06-01 |
| 修稿时间： | 2005年6月1日 |
The Improvement on the Method of Eletrophoretic Focusing |
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| XIE Yong-fang,LIANG Yi-long,ZHANG Yun,QIN Yan-jun,WU Wen-yong,HE Yuan-fang.The Improvement on the Method of Eletrophoretic Focusing[J].Letters in Biotechnology,2006,17(1):70-71. |
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| Authors: | XIE Yong-fang LIANG Yi-long ZHANG Yun QIN Yan-jun WU Wen-yong HE Yuan-fang |
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| Abstract: | Objective To introduce a simple efficient and fast method of isisoelectric focusing for isozyme analys. Methods It adopted two glass slides using two sides with glue agglutinate it. Cutting whatman as sample liquid paper. Results The improved methed is simple economical sensitive. There were more flexibility in applications higher resolution separations larger capacity of running samples shorter time for electrophoresis. Conclusion The method is feasible and efficient and worthy to be recommended. |
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| Keywords: | gel electrophoresis isoelectriphoresis focusing isoelctric point |
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