转瓶内部结构对无血清悬浮培养昆虫细胞的影响

以昆虫细胞为宿主进行基因工程产品的开发是动物细胞培养领域十分有吸引力的研究方向[1] 。由于昆虫细胞对营养要求极高 ,且对培养环境非常敏感 ,所以一般是在含有兼具营养及保护功能的胎牛血清的培养基中进行培养。血清一方面因其高额成本而限制了昆虫细胞大规模培养技术的发展 ,另一方面又因其成分复杂、富含蛋白而给外源基因表达产物的后处理带来困难。因此 ,昆虫细胞无血清培养技术的开发一直是细胞培养工程领域的研究热点 ,采用无血清培养技术取代传统的有血清培养技术已成为昆虫细胞 杆状病毒表达系统的发展趋势[2 ] 。然而 ,昆虫细…

Effect of Inner Designs of Spinner Flasks on Serum-free Cultivation of Insect Cells in Suspension

The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially designed spinner flask with relatively good culture environment,could support the fragile Tn5 cells to grow well even in low inoculum density.The effect of inner designs of spinner flasks on cellular growth and metabolism was discussed on the basis of this culture system.