Bubble coalescence behaviour in biological media |
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Authors: | I Adler J Diekmann W Hartke V Hecht F Rohn K Schügerl |
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Institution: | (1) Institut für Technische Chemie der Universität Hannover, Callinstr. 3, D-3000 Hannover, Federal Republic of Germany |
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Abstract: | Summary A twin bubble column was used to measure the kLa values for oxygen in model and cultivation media using the steady state method described previously (Adler et al. 1980). Desmophen and soy oil were used as antifoam agents together with model and/or cultivation media for Chaetomium cellulotyticum, Trichoderma reesei, Hansenula polymorpha, Saccharomyces cerevisiae and Escherichia coli. The bubble coalescence behavior is mainly influenced by antifoam agents and somewhat by protein and alcohol additives. In the range investigated (0.01 to 0.1%.), the kLa values are not influenced by the Desmophen concentration and only slighthly by the soy oil concentration (0.5 to 1.5%.). The coalescence behaviour was characterized by the ratio mcorr=(kLa)corr/(kLa)ref. A nutrient salt solution with Desmophen was used as a reference. The kLa measured in the investigated media were corrected by considering the differences in kLa's in the investigated and reference media. These mcorr values can directly be used for bubble columns close to the optimum aeration rate.Symbols a
Specific gas/liquid interfacial area
- c
Concentration
- kL
Mass transfer coefficient
- kLa
Volumetric mass transfer coefficient
- WSG
Superficial gas velocity
- EG
Relative gas hold-up |
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