Assisted assembly: how to improve a de novo genome assembly by using related species |
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| Authors: | Sante Gnerre Eric S Lander Kerstin Lindblad-Toh David B Jaffe |
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| Affiliation: | (1) Broad Institute of Harvard and MIT, Cambridge Center, Cambridge, Massachusetts 02142, USA;(2) Department of Medical Biochemistry and Microbiology, Uppsala University, Husarg.3, Uppsala, 751 23, Sweden |
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| Abstract: | We describe a new assembly algorithm, where a genome assembly with low sequence coverage, either throughout the genome or locally, due to cloning bias, is considerably improved through an assisting process via a related genome. We show that the information provided by aligning the whole-genome shotgun reads of the target against a reference genome can be used to substantially improve the quality of the resulting assembly. |
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