Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi |
| |
| Authors: | Abduvali Valiev Zumrut B Ogel Kier D Klepzig |
| |
| Institution: | (1) School of Forestry, Northern Arizona University, Flagstaff, AZ 86011, USA;(2) Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA;(3) Southern Research Station, USDA Forest Service, Pineville, LA 71360, USA;(4) Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA |
| |
| Abstract: | In this study, the production of extracellular enzymes by fungi associated with southern pine beetle was investigated for
the first time. Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic
symbiont Entomocorticium sp. A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures,
Entomocorticium sp. A showed maximum activity of 0.109 U/ml and 0.141 U/ml for total cellulase and endoglucanase activity respectively. Polyphenol
oxidase production was simultaneous with fungal growth. Characterization of polyphenol oxidase by activity staining suggests
that the enzyme is a tyrosinase/catechol oxidase. Enzyme assays in the presence of polyphenol oxidase inhibitors support the
results of the activity staining. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
