Application of electrophoretic methods for detection of protein-porphyrin complexes |
| |
Authors: | Kowalska Agnieszka Kwiek Piotr Mi?osz Ewa Gondek Grzegorz Romiszewska Anna Graczyk Alfreda Podhajska Anna J |
| |
Institution: | Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland. agak@biotech.univ.gda.pl |
| |
Abstract: | Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc(TM) 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|