Identification of some Bioactive Metabolites in a Fractionated Methanol Extract from Ipomoea aquatica (Aerial Parts) through TLC,HPLC, UPLC‐ESI‐QTOF‐MS and LC‐SPE‐NMR Fingerprints Analyses |
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| Authors: | Mahmoud Hefny Gad Emmy Tuenter Nagwa El‐Sawi Sabry Younes El‐Mewafy El‐Ghadban Kristiaan Demeyer Luc Pieters Yvan Vander Heyden Debby Mangelings |
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| Institution: | 1. Department of Analytical Chemistry and Pharmaceutical Technology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Brussels, Belgium;2. Medicinal and Aromatic Plants Research Department, Horticulture Institute, Agriculture Research Centre, Giza, Egypt;3. Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium;4. Department of Chemistry, Faculty of Science, Sohag University, Sohag, Egypt;5. Department of Toxicology, Dermato‐Cosmetology and Pharmacognosy, Centre for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Brussels, Belgium |
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| Abstract: | Introduction The plant species Ipomoea aquatica contains various bioactive constituents, e.g. phenols and flavonoids, which have several medical uses. All previous studies were executed in Asia; however, no reports are available from Africa, and the secondary metabolites of this plant species from Africa are still unknown. Objective The present study aims finding suitable conditions to identify the bioactive compounds from different fractions. Methodology Chromatographic fingerprint profiles of different fractions were developed using high‐performance liquid chromatography (HPLC) and then these conditions were transferred to thin‐layer chromatography (TLC). Subsequently, the chemical structure of some bioactive compounds was elucidated using ultra‐performance liquid chromatography‐quadrupole time of flight‐tandem mass spectrometry (UPLC‐QTOF‐MS) and liquid chromatography‐solid phase extraction‐nuclear magnetic resonance (LC‐SPE‐NMR) spectroscopy. Results The HPLC fingerprints, developed on two coupled Chromolith RP‐18e columns, using a gradient mobile phase (methanol/water/trifluoroacetic acid, 5:95:0.05, v/v/v), showed more peaks than the TLC profile. The TLC fingerprint allows the identification of the types of chemical constituents, e.g. flavonoids. Two flavonoids (nicotiflorin and ramnazin‐3‐O‐rutinoside) and two phenolic compounds (dihydroxybenzoic acid pentoside and di‐pentoside) were tentatively identified by QTOF‐MS, while NMR confirmed the structure of rutin and nicotiflorin. Conclusion The HPLC and TLC results showed that HPLC fingerprints give more and better separated peaks, but TLC helped in determining the class of the active compounds in some fractions. Bioactive constituents were identified as well using MS and NMR analyses. Two flavonoids and two phenolic compounds were tentatively identified in this species for the first time, to the best of our knowledge. Copyright © 2017 John Wiley & Sons, Ltd. |
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| Keywords: | Ipomoea aquatica chromatographic fingerprint analysis HPLC TLC UPLC‐QTOF‐MS LC‐SPE‐NMR |
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