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In vitro biosynthesis of juvenile hormone in larval honey bees: Comparison of six media
Authors:Anna Rachinsky  Klaus Hartfelder
Affiliation:1. USDA Bee Research Laboratory, 20705, Beltsville, Maryland
2. LS Entwicklungsphysiologie, Institut für Zoologie, Universit?t Tübingen, D-72076, Tübingen, Germany
Abstract:Summary We have formulated a tissue culture medium based on the components of larval honey bee hemolymph. Using an in vitro radiochemical assay to measure juvenile hormone biosynthesis, we compared our larval-based medium to four commercially available media (Grace’s, Medium-199; Shields and Sang M3, and Minimum Essential Medium), and a medium based on adult honey bee hemolymph. All media were formulated without methionine. There was no significant difference in the amounts of juvenile hormone produced by the larval medium and Grace’s; both of these media, however, were more suitable than the remaining four. Our larval-based tissue culture medium should prove useful in studies aimed at elucidating the underlying hormonal mechanism(s) of caste development in honey bees.
Keywords:corpora allata  radiochemical assay  insect organ culture  casts development  Hymenoptera   Apis mellifera
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