Measurement of oxygen concentration gradients in gel-immobilized recombinantEscherichia coli |
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Authors: | Christine M Hooijmans Catherine A Briasco Jun Huang Bert G M Geraats Jean-Noël Barbotin Daniel Thomas Karel Ch A M Luyben |
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Institution: | (1) Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands;(2) Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206 Compiègne, France |
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Abstract: | Summary In this study, an oxygen microsensor was used to measure oxygen concentration profiles in carrageenan gel particles containing growing, immobilizedEcherichia coli B (pTG201). Profiles, which were measured at intervals during continuous culture of gel slabs and beads, became increasingly steep with time. The oxygen penetration depth in the gel decreased with time, eventually reaching a steady state value of approximately 100 m for both gel beads and slabs. A reaction-diffusion model employing zero-order cell growth kinetics was found to provide an excellent fit to the experimental concentration data. Growth rates estimated from profiles obtained during the first few hours of culture were 0.24h–1 (gel slabs) and 0.18 h–1 (beads), compared to a value of 0.30 h–1 measured in free-cell suspensions at 25° C. |
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